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Fig. 7 | BMC Pulmonary Medicine

Fig. 7

From: A novel circ_MACF1/miR-942-5p/TGFBR2 axis regulates the functional behaviors and drug sensitivity in gefitinib-resistant non-small cell lung cancer cells

Fig. 7

Circ_MACF1 overexpression affects the functional behaviors and gefitinib sensitivity of A549/GR and PC9/GR cells by inducing TGFBR2 expression. A549/GR and PC9/GR cells were transfected with negative control Vector plasmid, circ_MACF1 overexpression plasmid, circ_MACF1 overexpression plasmid + si-NC, or circ_MACF1 overexpression plasmid + si-TGFBR2, followed by the assessment of TGFBR2 protein level by western blot (A), IC50 value for gefitinib by MTT assay (B), cell proliferation by EdU assay (C), cell colony formation by colony formation assay (D), cell proliferation by MTT assay (E, F), cell apoptosis by flow cytometry (G), cell migration and invasion by transwell assay (H, I), Twist1 and E-cadherin levels by western blot (J, K). *P < 0.05

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