Fig. 8

Overexpression of circ_MACF1 inhibits tumor growth under gefitinib in vivo. A qRT-PCR analysis of circ_MACF1 expression in A549/GR cells transduced by lentiviruses expressing circ_MACF1 (lenti-circ_MACF1) or a scrambled control (lenti-NC). A549/GR cells transduced by lenti-circ_MACF1 or lenti-NC were implanted into the right flanks of BALB/c nude mice (6 mice per group), with gefitinib administration (30 mg/kg) per os and tumor volume measurement (B) 10 days after implantation, and checked for tumor weight and images (C), circ_MACF1 expression by qRT-PCR analysis (D), miR-942-5p expression by qRT-PCR analysis (E), TGFBR2 mRNA level by qRT-PCR analysis (F), TGFBR2 protein level by western blot (G), TGFBR2 protein level and Ki67 staining by immunohistochemistry (H) at the end point (30 days after injection). *P < 0.05