Fig. 1
Apoptosis and Total ubiquitinated proteins expression in BPD rats. Neonatal rats were killed at P 7 and P14; normoxia group: 21% O2, hyperoxia group: 80–85% O2. A HE staining assay were used to observe the morphological changes of lung tissue and showed a significant decrease in radial alveolar count (Scale bar = 10 µm; Original magnifications: × 100). B TUNEL assay demonstrated a significant increase in the apoptosis index, in a pattern similar to that observed by immunofluorescence staining (Scale bar = 20 µm; Original magnifications: × 200; square frame magnification: × 400). C The expression of ubiquitinated proteins were detected by Western blot assays in the four groups and the grouping of blots cropped from the same gel. β-actin was used as the loading control. The part of original data for were presented in Additional file 1: Fig. S1. D Reagent kit with specific fluorescent substrate was utilized to measure the activity of 20 s proteasome. Values represent mean ± SD; ** P < 0.05 versus normoxia group