Fig. 3

Effects of the protein expression of GRP-78, PERK, ATF4, ATF6 and CHOP in AECII after hyperoxia exposure. A Western blot analysis of the AECII that were exposed to 95% oxygen (hyperoxia) or not (normoxia) for 24, 48 and 72 h, 30 g of total protein per well were loaded and subjected to Western blot analysis with corresponding antibodies shown on the left. The part of original data were presented in Additional file 1: Fig. S3. B–F Quantification of signals on the Fig. 3A, the results showed an increase in GRP78, PERK, ATF4, ATF6 and CHOP protein expression in hyperoxia group in comparison with normoxia group in 24, 48, and 72 h. Values are presented as mean ± SD; ** P < 0.05 versus normoxia group