Identification and detection of pathogenic bacteria from patients with hospital-acquired pneumonia in southwestern Iran; evaluation of biofilm production and molecular typing of bacterial isolates

Table 1 The primers oligonucleotide sequences used in this study

Target gene	Primer sequence (5′ → 3′)	Amplicon Length, bp	References
nucA	F-CTG GCA TAT GTA TGG CAA TTG TT R-TAT TGA CCT GAA TCA GCG TTG TCT	670	[23]
aroE	F-AAGGTGCGAATGTGACGGTG R-AACTGGTTCTACGTCAGGCA	620	[24]
ure-D	F-CCC GTT TTA CCC GGA AGA AG R-GGA AAG AAG ATG GCA TCC TGC	243	[25]
OprL	F-TTCCGGTGAAGGTGCCAAT R-ACCGGACGCTCTTTACCATA	507	Unpublished data
gltA	F-GACATCAACCACCGCGAAAT R-GGACCCCAAAGAGCAGAGAT	332	Unpublished data
mecA	F-GTG AAG ATA TAC CAA GTG ATT R-ATG CGC TAT AGA TTG AAA GGA T	147	[23]
SCCmec I	F-GCTTTAAAGAGTGTCGTTACAGG R-GTTCTCTCATAGTATGACGTCC	613	[26]
SCCmec II	F-CGTTGAAGATGATGAAGCG R-CGAAATCAATGGTTAATGGACC	398	[26]
SCCmec III	F-CCATATTGTGTACGATGCG R-CCTTAGTTGTCGTAACAGATCG	280	[26]
SCCmec IVa	F-GCCTTATTCGAAGAAACCG R-CTACTCTTCTGAAAAGCGTCG	776	[26]
SCCmec IVb	F-TCTGGAATTACTTCAGCTGC R-AAACAATATTGCTCTCCCTC	493	[26]
SCCmec IVc	F-ACAATATTTGTATTATCGGAGAGC R-TTGGTATGAGGTATTGCTGG	200	[26]
SCCmec IVd	F-CTCAAAATACGGACCCCAATACA R-TGCTCCAGTAATTGCTAAAG	881	[26]
SCCmec V	F-GAACATTGTTACTTAAATGAGCG R-TGAAAGTTGTACCCTTGACACC	325	[26]
agr I	R-GTCACAAGTACTATAAGCTGCGAT	440	[27]
agr II	R-GTATTACTAATTGAAAAGTGCCATAGC	573	[27]
agr III	R-CTGTTGAAAAAGTCAACTAAAAGCTC	406	[27]
agr IV	R-CGATAATGCCGTAATAC CCG	588	[27]
pan-agr	F-ATGCACATGGTGCACATGC		[27]

ISSN: 1471-2466