Fig. 1

Representative images of gross pathology: severe lung injury occurred in group H and group D, and the lung injury in group T was significantly less than that in group H (a); the levels of wet to dry weight ratio (W/D) (b); the protein level in BALF (c); representative appearances and photomicrographs of hematoxylineosin stained lung sections (magnification × 200 and × 400): severe lung injury occurred in group H and group D, and the lung injury in group T was significantly less than that in group H. Lung histology was characterized by perivascular edema, interstitial and intra-alveolar leukocyte infiltration, and marked heterogeneity in alveolar inflation (d); histological sub-scores in experimental groups: lung injury scores were determined based on leukocyte infiltration, exudative edema, hemorrhage, and alveolar wall thickness (e). C: Control group, Tidal volum e(TV): 0; L:l ow tidal volume group, VT: 7 ml/kg; H: High tidal volume group, TV: 30 ml/kg; D: DMSO group, TV: 30 ml/kg, Half an hour before making the model, the DMSO was injected; T: T-5224 group, TV: 30 ml/kg, Half an hour before making the model the T-5224 was injected, T1 (0. 3 mg/kg), T2 (3 mg/kg). Data are presented as the mean ± SD (n = 6Rat/group), *P < 0. 0001 versus C;*P < 0. 05 versus C; ####P < 0. 0001 versus H; ###P < 0. 001 versus H: ##P < 0. 01 versus H