Fig. 2

Knockdown of OSBPL5 inhibits cell proliferation, migration and invasion in NSCLC. A The knockdown efficiency of OSBPL5 in NSCLC cells was examined by qRT-PCR. *P < 0.05 vs. sh-NC group. B Western blot analysis for analyzing sh-OSBPL5#1/2 transfection efficiency in A549 and SPC-A1 cells. *P < 0.05 vs. sh-NC group. C, D Cell viability and proliferation in A549 and SPC-A1 cells after transfection of sh-NC, sh-OSBPL5#1, or sh-OSBPL5#2 were estimated via CCK-8 and EdU assays, respectively. *P < 0.05 vs. sh-NC group. E, F Cell migration and invasion in A549 and SPC-A1 cells of the three groups were detected by Transwell assays. *P < 0.05 vs. sh-NC group. G The levels of EMT process-related proteins (E-cadherin, N-cadherin and Vimentin) in A549 and SPC-A1 cells after transfection of indicated plasmids were evaluated by western blot analysis. *P < 0.05 vs. sh-NC group