Fig. 3

OSBPL5 is a downstream target of miR-526b-3p. A Six candidate OSBPL5-binding miRNAs were found with starBase database (condition: PicTar + microT). B The expressions of candidate miRNAs in NSCLC tissues and adjacent normal tissues was detected with qRT-PCR analysis. *P < 0.05 vs. Normal group. C The effect of the three miRNAs on the expression of OSBPL5 was detected with qRT-PCR analysis. *P < 0.05 vs. NC mimics. D The expression of miR-526b-3p in NSCLC cell lines (A549, SPC-A1, H1975 and PC-9) and normal human bronchial epithelial cell line (16HBE) was evaluated by qRT-PCR. *P < 0.05 vs. 16HBE group. E The overexpression effect of miR-526b-3p was tested by qRT-PCR. *P < 0.05 vs. NC mimics group. F The binding of miR-526b-3p with OSBPL5 was verified by luciferase reporter assay. *P < 0.05 vs. NC mimics group. G The influence of miR-526b-3p on the protein level of OSBPL5 was assessed by western blot analysis. *P < 0.05 vs. NC mimics group. H The correlation between miR-526b-3p and OSBPL5 was explored by Spearman’s correlation analysis