Fig. 3

ZIP12 knockdown decreased the proliferation and migration of MCT-PAH-PASMCs. MCT-PAH-PASMCs were infected with LV-shNC or LV-shZIP12. After 72 h of infection, the cells were serum starved for 24 h in 0.2% FBS in DMEM-F12, followed by restimulation with 10% FBS in DMEM-F12 for 48 h. A The proliferation of MCT-PAH-PASMCs in response to ZIP12 knockdown was determined by EdU assay (magnification, ×100; n = 6 independent experiments). B The migration of MCT-PAH-PASMCs in response to ZIP12 knockdown was determined by wound healing assay. The wounds were imaged every 24 h (magnification, ×40; n = 5 independent experiments). C, D Representative Western blot and summarized data of ZIP12 silencing and the effect on C PCNA and D cyclin D1 protein expression (n = 4 independent experiments). Ctrl control, PAH pulmonary arterial hypertension, PASMCs pulmonary arterial smooth muscle cells, EdU 5-ethynyl-2′deoxyuridine, MCT monocrotaline. The data are expressed as the mean ± standard deviation. Error bars represented standard deviation. *P < 0.05, **P < 0.01