Fig. 5

Overexpression of ZIP12 promoted the phosphorylation of AKT and ERK1/2 in Ctrl-PASMCs. A, B Ctrl-PASMCs were infected with LV-NC or LV-ZIP12. After 72 h of infection, cells were serum starved for 24 h in 0.2% FBS/DMEM-F12, followed by restimulation with 10% FBS/DMEM-F12 for 1 h. Representative Western blot and summarized data showing the protein expression of A phosphorylated and total AKT and B phosphorylated and total ERK1/2 in Ctrl-PASMCs after ZIP12 overexpressing (A, n = 4 independent experiments; B, n = 4 independent experiments). C, D Ctrl-PASMCs were infected with LV-NC or LV-ZIP12. After 72 h of infection, cells were serum starved for 24 h in 0.2% FBS/DMEM-F12, and then pretreated with 10 μM LY294002 or 10 μM U0126 for 30 min prior to restimulation with 10% FBS/DMEM-F12 for 1 h. Representative Western blot and summarized data showing the protein expression of C phosphorylated and total AKT and D phosphorylated and total ERK1/2 in ZIP12-overexpressing Ctrl-PASMCs treated with either ERK inhibitor U0126 or AKT inhibitor LY294002, or untreated (C, n = 6 independent experiments; D, n = 6 independent experiments). Ctrl control, PASMCs pulmonary arterial smooth muscle cells. The data are expressed as the mean ± standard deviation. Error bars represented standard deviation. **P < 0.01