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Fig. 3 | BMC Pulmonary Medicine

Fig. 3

From: Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure

Fig. 3

Regulation of Notch signaling induces endothelial cell (ECs) differentiation. A, B Versus that in the control group (11.32 ± 1.27%), the highest percentage of CD31 + cells (16.18 ± 2.03%) was observed in the N1ICD overexpression group; that in the CSE group was 5.75 ± 0.82% and that in the Notch-shRNA2 group was 1.09 ± 0.33%. C–F After 14 days of induction, VEGFR2 protein expression was determined by western blotting assays and eNOS and VE-cadherin transcript levels were determined by quantitative RT-PCR; N1ICD overexpression rescued the downregulation of VEGFR2, eNOS, and VE-cadherin expression induced by 2%CSE treatment, which were significantly decreased in GV-248-Notch1-shRNA2 transfected mesenchymal stem cells (MSCs). *P < 0.05 vs. control group, #P < 0.05 vs. CSE group; CSE, cigarette smoke extract

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BMC Pulmonary Medicine

ISSN: 1471-2466

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