Skip to main content
Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure

Fig. 4

Notch signaling via RBP-Jκ promotes Jagged-1 expression. Jagged-1 enhanced the transcriptional activation of RBP- Jκ, which was endogenous Notch signaling-dependent. A 2%CSE treatment inhibited Notch1 reporter activation. Incubation with Jagged1 Fc significantly enhanced Notch1 reporter activation by greater than 8.1-fold relative to that in vehicle-treated mesenchymal stem cells (MSCs), whereas this improvement was suppressed by Notch-shRNA; Jagged1/Notch signaling also upregulated Jagged1 protein expression in MSCs and this response was blocked by Notch1-shRNA2 accordingly B, C *P < 0.05 vs. control group, #P < 0.05 vs. CSE group; CSE, cigarette smoke extract. D MSCs, Lenti-V-MSCs, and N1ICD over-expressing MSCs were co-cultured with MSCs expressing a Notch reporter gene 24 h after transfection. E After 24 h of co-culture, Notch reporter expression was significantly increased in N1-ICD-overexpressing MSCs in the lower compartment relative to that in control co-cultures, whereas a significant decrease was observed when the lower compartment cells were pre-transfected with RBP-Jκ shRNA. F, G Jagged1 protein expression in lower compartment cells was determined by western blotting, and these cells exhibited similar expression trends with Notch reporter expression. *P < 0.05

Back to article page