Fig. 2

Criteria for selecting a number of categories and consensus matrix for DNA methylation classification with the corresponding heat map. A The consensus among clusters for each category number k. B Delta area curves for consensus clustering indicating the relative change in area under the CDF curve for each category number k compared to k − 1. The horizontal axis represents the category number k and the vertical axis represents the relative change in area under the CDF curve. C Color-coded heatmap corresponding to the consensus matrix for k = 7 obtained by applying consensus clustering. Color gradients represent consensus values from 0 to 1; white corresponds to 0 and dark blue to 1. D A heatmap corresponding to the dendrogram in C was generated using the heatmap function with DNA methylation classification, TNM stage, clinicopathological stage, and histological type as the annotations