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Fig. 2 | BMC Pulmonary Medicine

Fig. 2

From: Effects of SOCS1-overexpressing dendritic cells on Th17- and Treg-related cytokines in COPD mice

Fig. 2

SOCS1 gene overexpression lentivirus transfection of DCs. a pLVX-IRES-ZsGreen1 vector. b PG-p1-VSVG. c PG-P2-REV. d PG-P3-RRE. e pLVX-SOCS1-IRES-ZsGreen and negative control lentivirus titer assay results. f Fluorescence microscopic observation of SOCS1 gene overexpression transfected DCs (DCs-Ad-SOCS1 group) and empty GFP lentivirus transfected DCs (DCs-Ad-GFP group) at 5 days of incubation. g DNA content of DCs-Ad-SOCS1, DCs-Ad-GFP, and imDCs groups. h WB bands. i WB grayscale analysis

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