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Fig. 1 | BMC Pulmonary Medicine

Fig. 1

From: Salt-inducible kinase 2 (SIK2) inhibitor ARN-3236 attenuates bleomycin-induced pulmonary fibrosis in mice

Fig. 1

Inactivation of SIK2 led to the dephosphorylation and nuclear translocation of CRTC2 in human fetal lung fibroblasts (HFLs). HFLs or SIK2 overexpressed HFLs (HFLs-SIK2) were treated with DMSO (0.1%), ARN-3236 (0.5 μM) or transfected with SIK2 SiRNA (100 nM). Cells were then treated with TGF-β1 (5 ng/mL) for 12 h. A, B Representative western blot bands and quantitation of SIK2, pCRTC1, pCRTC2 and CRTC2 protein expressions in HFLs. C Representative confocal images and quantification of the nuclear translocated CRTC2 in HFLs and HFLs-SIK2 cells. Data are expressed as mean ± SEM of at least 4 independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05

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