Fig. 6

Flow cytometric analysis of Th2 cells in lung and BALF. a The protocol for gating Th2 cells (CD4⁺GATA3⁺). b Flow cytometric identification of Th2 cells in single-cell suspensions of the BAL fluid (BALF) and lung parenchyma (Lung) of WT and Il9^−/−mice challenged with HDM and NS. Left: representative plots of CD4⁺GATA3⁺ Th2 cells of indicated mice. Right: the percentages of Th2 cells in lymphocytes from BALF and lung. And total number of Th2 cells in BALF and lung. c Flow cytometric analysis of Th2 cells expressing Ki67 in lung parenchyma. Left: representative plots of Ki67⁺Th2 cells of indicated mice. Right: the percentages and absolute number of Ki67⁺Th2 cells in lung parenchyma. Bars show the mean ± SEM (n = 3 in each group). *p < 0.05, **p < 0.01, ***p < 0.001