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Fig. 4 | BMC Pulmonary Medicine

Fig. 4

From: C1QTNF6 regulated by miR‐29a-3p promotes proliferation and migration in stage I lung adenocarcinoma

Fig. 4

Relationship between miR‐29a-3p and C1QTNF6 was confirmed. A The upstream regulators of C1QTNF6 were discovered by a prediction website. B Correlations between expression of miR‐29a‐3p and C1QTNF6 in TCGA database. C The expression of miR‐29a‐3p in stage I LUAD and paired adjacent tissues was detected by RT-qPCR. D Kaplan‐Meier survival analysis of miR‐29a‐3p based on the TCGA LUAD data. E MiR-29a-3p expressions of H1975 and A549 cells after suppressing C1QTNF6 was evaluated using RT-qPCR. F C1QTNF6 mRNA expressions of H1975 and A549 cells after overexpression or knockdown of miR-29a-3p was evaluated using RT-qPCR. (G-H) C1QTNF6 protein expressions of H1975 and A549 cells after overexpression or knockdown of miR-29a-3p was evaluated using western blot. I Schematic representation of miR-29a-3p binding sites in the C1QTNF6 3'UTR. J The reporter activity was detected by dual-luciferase reporter system, luciferase reporter activity is normalized to that of renilla luciferase. miR‐29a-3p, miR‐29a-3p mimics; NC, negative control; wt: wide type; mut: mutated. n = 3. (Data were presented as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001)

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