Fig. 4

Linc00511 interacts with miR-126-5p and miR-218-5p. A After Linc00511 knockdown, the levels of nine differentially expressed miRNAs in lung adenocarcinoma were determined by RT–qPCR. B The potential targeting sequences of miR-126-5p and miR-128-5p in Linc00511 were predicted, and a schematic of the wild-type and mutant Linc00511 constructs is shown. C Dual luciferase reporter vectors were used to test whether Linc00511 directly binds to miR-126-5p and miR-218-5p. Luciferase activity was measured in A549 cells cotransfected with Linc00511-WT or Linc00511-MUT and the miR-126-5p mimic or miR-218-5p mimic. D An RNA pulldown assay revealed the interactions of Linc00511 with miR-126-5p and miR-218-5p. E Measurement of miR-126-5p and miR-218-5p mRNA levels in BEAS-2B, A549, and PC9 cells. **P < 0.01, ***P < 0.001 versus the si-nc, miR-NC, BEAS-2B, or Biotin-nc group