Fig. 5

Linc00511 regulates COL1A1 expression by targeting miR-126-5p or miR-218-5p. A The Venn diagram shows the overlapping genes coregulated by miR-126-5p and miR-218-5p that were predicted by the TargetScan and starBase databases. B Gene ontology (GO) enrichment analysis of miR-126-5p and miR-218-5p target genes. C Prediction of miR-126-5p and miR-218-5p binding sites in the COL1A1 mRNA 3’-UTR. D Dual luciferase reporter vectors were used to verify the interactions of miR-126-5p and miR-218-5p with COL1A1. E An RNA pulldown assay confirmed the relationships between COL1A1 and both miR-126-5p and miR-218-5p. F The relative COL1A1 expression levels were examined in BEAS-2B, A549 and PC9 cells by RT–qPCR. G COL1A1 mRNA and H protein expression in A549 cells transfected with si-linc00511, anti-nc + si-linc00511, anti-miR-218-5p + si-linc00511, or anti-miR-126-5p + si-linc00511. **P < 0.01, ***P < 0.001 versus the miR-NC, BEAS-2B or Biotin-nc group; ^#P < 0.05 versus the si-linc00511 + anti-nc group