Fig. 6

miR-125b-5p was the target of lncRNA AK089514. A Venn diagram showing 82 putative miR-125b-5p target lncRNAs predicted by TargetScan algorithms and upregulated in M1 macrophages. B The 7 key candidate lncRNAs was obtained by re-annotation. C RT-qPCR was used to detected the lncRNAs level in nuclear and cytoplasmic fractions from BMDMs. U6 and GAPDH were acted as the nuclear and cytoplasmic controls, respectively. D RNA FISH was used to detect the expression of lncRNA AK089514 in BMDMs. E Putative binding sequence between lncRNA AK089514 and miR-125b-5p. F Luciferase reporters containing WT or MUT lncRNA AK089514 transcript were co-transfected with miR-125b-5p mimics or negative control. Data are mean ± SD from three independent experiments. ** P < 0.01