Table 2 Technical details of the PCR methods used in the studies included

Subhagan [20]

1

Proteinase

Phenol–chloroform

Pan Aspergillus

18S rRNA

Mikulska [21]

0.2

QIAamp DSP virus spin kit

QIAamp

Multiplex real-time

28S rRNA

Scharmann [22]

0.5

Maxwell16 tissue LEV total DNA/RNA purification kit

Maxwell16 tissue LEV total DNA/RNA purification

MycoGENIE or Fungiplex or AsperGenius real-time

28S rRNA

Unterman [23]

Unknown

QIAamp DNA mini Kit

QIAamp

Nested pan-Aspergillus

18S rRNA

Pelzer [24]

1

Maxwell16 DNA kit

Maxwell16

AsperGenius real-time

Aspergillus-species

Mikulska [25]

0.5

MycoGENIE DNA extraction kit

MycoGENIE

MycoGENIE real-time

28S rRNA

Hardak [26]

5

Proteinase

QIAamp

Nested

18S rRNA

Wehrle-Wieland [27]

0.2

Proteinase

EZ1 DNA tissue kit

In-house

ITS1-5.8S rRNA

Prattes [28]

0.4

NucliSens easyMAG

NucliSens easyMAG

MycoGENIE real-time

28S rRNA

Heldta [29]

1

Proteinase

Phenol–chloroform

Nested

Aspergillus-species

Guegan [30]

1

Proteinase

QIAamp

In-house

28S rRNA

Grancini [31]

0.2

Proteinase

EZ1 DPS virus kit

Real-time

rDNA18S

Denis [32]

0.2

QIAamp DNA mini kit

QIAamp

Real-time

ITS1 region or 28S rRNA

Boch [33]

1.5

Proteinase

Phenol–chloroform

Nested

18S rRNA

Montesinos [34]

0.8

QIAsymphony DSP virus/pathogen midi kit

QIAsymphony DSP virus/pathogen midi kit

Real-time

Aspergillus-species

Eigl [35]

1.5

Proteinase

Phenol–chloroform

Nested

18S rRNA

Bhimji [36]

Unknown

Unknown

Unknown

Droplet digital

Pan-Aspergillus

Zhang [37]

1

Glass beads

DNeasy plant mini kit

Real-time

28S rRNA

Chong [38]

1

Proteinase

NucliSENS miniMAG

AsperGenius real-time

28S rRNA

Boch [39]

Unknown

Proteinase

Phenol–chloroform

Nested

18S rRNA

Chong [40]

1

Proteinase

NucliSENS miniMAG

AsperGenius real-time

28S rRNA

Hoenigl [41]

1.5

Proteinase

Phenol–chloroform

Nested

18S rRNA

Heng [42]

0.6

GeneElute mammalian DNA extraction kit

GeneElute mammalian DNA extraction kit

Nested

18S rRNA

Reinwald [43]

1.5

Proteinase

Phenol–chloroform

Nested

18S rRNA

Reinwald [44]

1.5

Proteinase

Phenol–chloroform

Nested

18S rRNA

Buess [45]

2.5

Proteinase

EZ1 DNA tissue Kit

Nested

18S rRNA

Torelli [46]

2

Glass beads

MycXtra fungal DNA

Real-time

18S rRNA

Luong [47]

0.5

Glass beads

AllPrep DNA/RNA mini kit

Real-time

Pan-Aspergillus

Hadrich [48]

0.2

Proteinase

QIAamp

Real-time

18S rRNA

Fréalle [49]

0.2

QIAamp DNA mini kit

QIAamp

Real-time

Unknown

Shahid [50]

0.4

Proteinase

Phenol–chloroform

End-point

Aspergillus-species

Khot [51]

2–5

MasterPure yeast DNA kit

MasterPure yeast DNA kit

Real-time

18S rRNA

Musher [52]

0.5

Proteinase

MasterPure yeast DNA kit

Real-time

18S rRNA

Sanguinetti [53]

1.5

DNeasy plant mini kit

DNeasy plant mini kit

Real-time

18S rRNA

Rantakokko [54]

1.5

Proteinase

Phenol–chloroform

Real-time

mtDNA

Raad [55]

1

Proteinase

Phenol–chloroform

End-point

alkaline protease mtDNA

Hayette [56]

0.5

Proteinase

Phenol–chloroform

Nested

alkaline protease mtDNA

Buchheidt [57]

1.5

Lyticase

Phenol–chloroform

Nested

18S rRNA

Jones [58]

0.2

Proteinase

Phenol–chloroform

PCR-ELISA

mtDNA

Bretagne [59]

1.5

Proteinase

Phenol–chloroform

Competitive

mtDNA

Tang [60]

0.25

Proteinase

Phenol–chloroform

End-point

alkaline protease mtDNA