Fig. 3

PKD2 alleviated LPS-induced inflammatory injury in the lung. Mice were intratracheally injected with adenovirus expressing PKD2 or GFP (5 × 10¹² vg/ml) in 30 µl of PBS for three days. (A and B) The transfection efficiency was detected by fluorescence microscopy (scale bar: 100 μm), and the expression of PKD2 was determined by western blotting. Mice were intratracheally injected with adenovirus expressing PKD2 or GFP (5 × 10¹² vg/ml) in 30 µl of PBS for three days. Then, the mice were intratracheally injected with LPS (1 mg/kg) for 24 h or intraperitoneally injected with the autophagy inhibitor 3-MA (15 mg/kg) for 30 min, followed by LPS (1 mg/kg) treatment for 24 h. (C) Histological evaluation of the lung samples was carried out using HE staining. (D) Lung injury scores were determined on the basis of four independent parameters. (E) The lung W/D ratio was detected. (F) MPO activity in the lung tissues was detected with an MPO activity kit. The experiments were repeated three times (three independent experiments). The data shown in the graphs are the mean ± SEM. *P < 0.05