Lefamulin efficacy and safety in a pooled phase 3 clinical trial population with community-acquired bacterial pneumonia and common clinical comorbidities

Background Lefamulin, a first-in-class pleuromutilin antibiotic approved for intravenous and oral use in adults with community-acquired bacterial pneumonia (CABP), was noninferior to moxifloxacin in the Lefamulin Evaluation Against Pneumonia (LEAP) 1 intravenous-to-oral switch study and the LEAP 2 oral-only study. Using pooled LEAP 1/2 data, we examined lefamulin efficacy/safety overall and within subgroups of patients presenting with comorbidities typical in CABP management. Methods In LEAP 1, adults with CABP were randomized to receive intravenous lefamulin (150 mg every 12 h) for 5‒7 days or moxifloxacin (400 mg every 24 h) for 7 days, with optional intravenous-to-oral switch if predefined improvement criteria were met. In LEAP 2, adults with CABP were randomized to receive oral lefamulin (600 mg every 12 h) for 5 days or moxifloxacin (400 mg every 24 h) for 7 days. Both studies assessed early clinical response (ECR) at 96 ± 24 h after first study drug dose and investigator assessment of clinical response (IACR) at test-of-cure (5‒10 days after last dose). Pooled analyses of the overall population used a 10% noninferiority margin. Results Lefamulin (n = 646) was noninferior to moxifloxacin (n = 643) for ECR (89.3% vs 90.5%, respectively; difference  − 1.1%; 95% CI  − 4.4 to 2.2); IACR success rates at test-of-cure were similarly high (≥ 85.0%). High efficacy with both lefamulin and moxifloxacin was also demonstrated across all well-represented patient subgroups, including those with advanced age, diabetes mellitus, a history of cardiovascular diseases (e.g., hypertension, congestive heart failure, or arrhythmia) or chronic lung diseases (e.g., asthma or chronic obstructive pulmonary disease), elevated liver enzymes, or mild-to-moderate renal dysfunction. No new safety signals were identified. Conclusions Lefamulin may provide a valuable intravenous/oral monotherapy alternative to fluoroquinolones or macrolides for empiric treatment of patients with CABP, including cases of patients at risk for poor outcomes due to age or various comorbidities. Trial registration ClinicalTrials.gov LEAP 1 (NCT02559310; Registration Date: 24/09/2015) and LEAP 2 (NCT02813694; Registration Date: 27/06/2016). Supplementary Information The online version contains supplementary material available at 10.1186/s12890-021-01472-z.

Background Pneumonia, a leading cause of US hospitalizations and infection-related deaths [1][2][3], is associated with substantial morbidity and mortality [4]. Pneumonia-associated economic burden is considerable, with annual costs estimated at €10.1 billion in Europe [5] and exceeding $17 billion in the United States [6]. Pneumonia prognoses can range from rapid resolution to death [7], and clinical management of community-acquired bacterial pneumonia (CABP) can be complicated by many factors [1]. CABP incidence and impact are greater in older versus younger individuals, and older patients with CABP often present with challenges (e.g., increased resistance rates, polypharmacy/drug interactions) that can impede treatment efficacy and safety [8,9]. Comorbidities such as chronic obstructive pulmonary disease (COPD), congestive heart failure (CHF), or diabetes mellitus (DM) increase the risk of severe CABP and may aggravate clinical symptoms and complicate management [9,10]. Underlying cardiac or liver disease also increase the risk of potential cardiac or liver toxicities, respectively, associated with CABP antimicrobials [11][12][13].
Increasingly, new CABP treatment options are needed owing to rising antibacterial resistance rates and undesirable risks/adverse effects associated with current treatments, including allergic reactions and fluoroquinolone-associated disability (e.g., tendon injury, aortic rupture, glucose homeostasis imbalance, and neurocognitive effects, especially in older patients) [1,11,[14][15][16]. Lefamulin, a first-in-class pleuromutilin antibiotic that selectively inhibits bacterial protein synthesis [17,18], is approved for intravenous (IV) and oral use in adults with CABP [19]. In adults with CABP, lefamulin demonstrated noninferiority to moxifloxacin in the IV-to-oral switch Lefamulin Evaluation Against Pneumonia (LEAP) 1 study [20] and the oral-only LEAP 2 study [21]. Lefamulin has demonstrated potent in vitro activity against a global collection of typical (eg, Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis) and atypical (e.g., Mycoplasma pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae) CABP-causative pathogens, including antimicrobialresistant strains [22,23]; lefamulin has also demonstrated clinical efficacy against these and other CABP pathogens [20,21]. Moreover, lefamulin has demonstrated weak activity against Bacteroides fragilis group strains and Enterobacteriaceae, suggesting decreased potential for disruption to gastrointestinal (GI) microbiota by IV lefamulin (Data on file, Nabriva Therapeutics, Vienna, Austria). In contrast to other common CABP therapies, the suitability of lefamulin for oral administration and its targeted antimicrobial activity [19] may position it as an attractive option for use in patients with advanced age, various comorbid conditions, increased disease severity, or drug allergies. Therefore, data from the 2 pivotal phase 3 LEAP studies in adults with CABP were pooled to further examine lefamulin efficacy and safety overall and within various patient subgroups.

Study design and participants
The LEAP 1 (NCT02559310; Registration Date: 24/09/2015) [20] and LEAP 2 (NCT02813694; Registration Date: 27/06/2016) [21] studies were double-blind, double-dummy, parallel-group trials evaluating lefamulin versus moxifloxacin in adults with moderate to severe CABP. Before study initiation, participating centers obtained study approval from appropriate ethics committees/institutional review boards (Additional file 1: Appendices 1-2), and patients provided written informed consent. Trials were compliant with the ethical principles of the Declaration of Helsinki, Good Clinical Practice guidelines, and local laws/regulations.
Detailed methods for both studies have been published elsewhere [20,21]. Briefly, randomization (1:1) was stratified in both studies by Pneumonia Outcomes Research Team (PORT) risk class (III vs IV/V [LEAP 1]; II vs III/IV [LEAP 2]), geographic region (US vs ex-US), and receipt of a single dose of a short-acting antibacterial for CABP before randomization (yes vs no). In LEAP 1, patients received IV lefamulin 150 mg every 12 h (q12h) for 5-7 days or moxifloxacin 400 mg every 24 h (q24h) for 7 days. If methicillin-resistant S. aureus (MRSA) was suspected at screening, blinded IV linezolid (600 mg q12h) was added to moxifloxacin, or linezolid placebo was added to lefamulin.
In LEAP 1, patients could switch to oral therapy (lefamulin 600 mg q12h or moxifloxacin 400 mg q24h) after 6 IV doses (~ 3 days) if predefined improvement criteria were met. The original study protocol indicated a 5-day lefamulin treatment period (10 days in patients with CABP due to L. pneumophila or MRSA or in patients with S. pneumoniae and bacteremia); however, this was later adjusted to 7 days (except in confirmed MRSA cases, which continued to receive 10 days of treatment) to reduce medication errors and limit study site burden. In LEAP 2, patients received oral lefamulin 600 mg q12h (5 days) or moxifloxacin 400 mg q24h (7 days).
Patients eligible for enrollment included adults with radiographically diagnosed pneumonia, PORT risk class III-V (LEAP 1) or II-IV (LEAP 2), acute onset of ≥ 3 CABP symptoms (e.g., dyspnea), ≥ 2 vital sign abnormalities (e.g., tachycardia), and ≥ 1 other clinical sign or laboratory finding of CABP (e.g., hypoxemia). Exclusion criteria included receipt of > 1 dose of a shortacting CABP oral/IV antibacterial within 72 h before randomization, being at risk for major cardiac events or dysfunction, significant hepatic disease, and severely impaired renal function. Of note, not all patients with comorbidities were excluded; patients with DM, asthma/ COPD, non-major cardiac events, elevated liver enzymes, and mild-to-moderate renal dysfunction were included and well represented in the LEAP 1 and LEAP 2 trials.

Study outcomes
In both studies, the US Food and Drug Administration (FDA) primary efficacy endpoint was early clinical response (ECR) at 96 ± 24 h after first study drug dose in the intent-to-treat (ITT) population (all randomized patients). Patients were programmatically classified as ECR responders if they were alive, showed improvement in ≥ 2 CABP symptoms, had no CABP symptom worsening, and received no nonstudy antibiotic for CABP treatment. Achievement of ECR criteria was also assessed at each study day, end of treatment (EOT; within 2 days after last study drug dose), test of cure (TOC; 5-10 days after last study drug dose), and late follow-up (LFU; days [27][28][29][30][31][32][33][34]. Secondary endpoints (European Medicines Agency primary endpoints) were investigator assessment of clinical response (IACR) at TOC in the modified ITT (mITT) and clinically evaluable (CE) populations. The mITT population included all randomized patients who received any study drug. The CE population comprised patients with no indeterminate clinical response, ≥ 48 h of study drug (unless patient died before 48 h), receipt of no nonstudy antibacterial potentially effective against CABP pathogens (unless administered for clinical failure), and no additional efficacy confounding factors. Investigator assessment classified patient response as a success if CABP signs/symptoms were resolved or improved such that no additional antibiotic was administered for the current CABP episode or as a failure if nonstudy antibiotics were administered or patient died from any cause. IACR success rates were also assessed at EOT and LFU.
Additional analyses included ECR and IACR assessments in subgroups based on baseline demographics and disease characteristics (Additional file 2: Table 1). Safety was assessed in all randomized patients who received any amount of study drug (safety population). Treatment-emergent adverse events (TEAEs) were defined as adverse events (AEs) that started/worsened during or after first study drug administration and were monitored at all study visits and by patient reporting. Additional safety assessments included vital signs, laboratory tests, and triplicate 12-lead electrocardiograms, which were performed within a 5-min interval at screening and on days 1 and 3 (LEAP 1) or days 1 and 4 (LEAP 2) to capture changes at the estimated maximum observed plasma concentration of study drug.

Statistical analyses
Pooled ECR and IACR analyses were evaluated using a 10% noninferiority margin; lefamulin noninferiority versus moxifloxacin was concluded if the lower 95% CI limit for the treatment difference exceeded − 10%. Treatment differences were weighted by study. The 95% CI was calculated using the Miettinen-Nurminen method [24], adjusted for study (ECR and IACR) and receipt of a prior single-dose short-acting antibiotic (IACR only), with the inverse variance of the effect size used for stratum weights. For subgroup analyses, descriptive CIs were determined but no noninferiority conclusions were made. If the treatment difference and CI were not estimable using these methods, observed treatment differences were reported and 95% CIs were calculated via unadjusted continuity-corrected Z-test.

Clinical response/success
In the pooled ITT population, ECR rates were high (lefamulin 89.3%; moxifloxacin 90.5%; difference − 1.1; 95% CI − 4.4 to 2.2), and lefamulin was noninferior to moxifloxacin (Fig. 1a). Most patients (> 60%) met ECR criteria by day 3, with > 80% of patients in both treatment groups meeting ECR criteria by day 4; further increases through day 7 and sustained efficacy through LFU were also observed ( Fig. 1b). Similarly, proportions of patients with File Jr. et al. BMC Pulm Med (2021) 21:154 resolution of all baseline CABP signs and symptoms also increased over time in both treatment groups (Fig. 2).
Lefamulin was also noninferior to moxifloxacin for IACR success (mITT and CE populations), with high and similar rates at TOC (Fig. 1c, d) for both lefamulin and moxifloxacin. IACR success rates at EOT and LFU were similarly high (> 83%) and consistent between treatment groups.
Lefamulin and moxifloxacin demonstrated high and similar ECR rates across various baseline demographic and disease characteristics, including patients with advanced age or comorbidities (asthma/COPD, DM, hypertension, CHF, arrhythmia, elevated liver enzymes) and regardless of PORT risk class (Fig. 3a). IACR success rates at TOC for both treatment groups were similarly high across most subgroups (Fig. 3b, c).

Safety
Within the pooled safety population (n = 1282), mean (SD) study drug exposure was 4.9 (1.5) days for lefamulin (n = 641) and 6.8 (1.1) days for moxifloxacin (n = 641) overall. This included patients who received IV treatment only (LEAP 1 patients; mean duration of 5. Overall TEAE rates were similar with lefamulin (34.9%) and moxifloxacin (30.4%; Table 2); most TEAEs were mild to moderate in severity and few patients (< 5%) experienced severe TEAEs. Treatment-related TEAEs were reported in 15.4% (lefamulin) and 10.6% (moxifloxacin) of patients, serious TEAEs in 5.6% and 4.8%, and TEAEs leading to study drug discontinuation in 3.1% and 3.3%. Over the entire study duration, 19 patients experienced TEAEs leading to death (lefamulin n = 11, moxifloxacin n = 8; Table 2). Among patients with common comorbidities, lefamulin and moxifloxacin demonstrated   Table 2), and most were mild to moderate in severity. Differences between the 2 treatment groups were driven primarily by GI events associated with oral dosing in the LEAP 2 study. Few patients discontinued study drug because of GI TEAEs (lefamulin, 0.5% [3/641]; moxifloxacin, 0.2% [1/641]). One case of Clostridium difficile infection was reported in a LEAP 2 patient successfully treated with lefamulin; onset occurred ~ 1 week after completing 5 days of lefamulin treatment, patient remained hospitalized, and infection resolved after oral vancomycin treatment.
Treatment-emergent adverse events (TEAEs) in the Medical Dictionary for Regulatory Activities system organ class (SOC) of cardiac disorders occurred in 2.5% (lefamulin) and 3.1% (moxifloxacin) of patients (Additional file 2: Table 4). After dosing, mean QT intervals corrected according to Fridericia (QTcF) increased from baseline in both treatment groups, although the mean (SD) maximum change from baseline was numerically smaller with lefamulin (16.9 [16.9]  of hypertension or arrhythmia and those aged ≥ 65 years, TEAEs in the cardiac disorders SOC were reported at similar and low (≤ 10%) incidences in both treatment groups. Patients who experienced an increase from baseline in QTcF > 60 ms or QTcF value > 500 ms were similarly few and more frequent with moxifloxacin than lefamulin. Among patients with elevated baseline liver enzymes and those aged ≥ 65 years, TEAEs in the hepatobiliary disorders SOC were similarly infrequent in both treatment groups (Additional file 2: Table 5). Within these patient subgroups, postbaseline elevated liver enzymes occurred in few patients, were similar with lefamulin and moxifloxacin, and resolved upon treatment discontinuation.

Discussion
This pooled analysis confirmed lefamulin clinical efficacy and its noninferiority to moxifloxacin for treatment of adults with CABP. Outcome rates were high with   Table 1 [25][26][27], complete resolution of all CABP clinical signs and symptoms lagged noticeably behind ECR and cessation of antimicrobial therapy, which may be attributed to the inflammatory nature of CABP and exacerbation of preexisting conditions (e.g., asthma/COPD) or smoking-associated disability [26]. Despite this lag, however, hospitalized patients in the LEAP studies achieved clinical improvement and stability by treatment day 3 and were discharge-ready (ie, improved in ≥ 2 cardinal CABP symptoms) by day 4 [28].
Lefamulin and moxifloxacin demonstrated high and similar ECR and IACR success rates across almost all patient subgroups, with a few exceptions that may be due to small sample sizes (e.g., patients with bacteremia) or unadjusted confounders (e.g., aged < 65 years, meeting American Thoracic Society minor criteria) [29]. Complex cases of patients at risk for poor efficacy or safety outcomes due to age or comorbidity were well represented, and subpopulation analyses demonstrated high lefamulin efficacy similar to that with moxifloxacin. Similar results were observed among patients with more severe disease (i.e., PORT risk class III-V), although clinical response rates were numerically higher in patients with PORT risk class III versus IV/V.
Lefamulin was generally well tolerated. Compared with moxifloxacin, lefamulin was associated with more frequent GI-related TEAEs; however, these were all nonserious, manageable, and rarely led to discontinuation. Patients aged ≥ 65 years or with hypertension, arrhythmia, or elevated liver enzymes are generally at greater risk of adverse safety outcomes, but these data demonstrate a safety profile for lefamulin in these patient subgroups comparable to that of the overall population. When stratified by PORT risk class, lefamulin and moxifloxacin had similar safety profiles, with higher TEAE and serious TEAE rates in patients with PORT risk class IV/V versus III, consistent with the older and more comorbid demographic of PORT risk class IV/V patients. Furthermore, the favorable safety profile observed in PORT risk class III patients suggests that they may be candidates for outpatient treatment, consistent with current consensus guidelines [30].
Extensive nonclinical testing of lefamulin suggested a potential for QT prolongation, and further assessment in phase 1 studies of healthy volunteers demonstrated dose/ exposure-related QT interval effects [19]. Consistent with these findings, mild QT prolongation was seen with clinically relevant lefamulin doses in this analysis, but observed effects were consistently numerically smaller than with moxifloxacin, although the implications of QT prolongation with lefamulin should continue to be examined in clinical practice. Lefamulin is not recommended for patients taking other drugs with known QT effects [19], and experts on long QT syndrome recommend performance of an electrocardiogram before and after starting therapy with any drug(s) that has a probable or possible association with QT prolongation [31].
Potential liver injury is a concern with multiple antibiotic classes, and most (eg, cephalosporins, fluoroquinolones, macrolides, tetracyclines) list hepatic enzyme elevation and other hepatic AEs in their package inserts [32][33][34]. Consistent with extensive nonclinical evaluations suggesting lefamulin is unlikely to result in Table 2 Overall summary of TEAEs (pooled safety population) AE adverse event, COPD chronic obstructive pulmonary disease, MedDRA Medical Dictionary for Regulatory Activities, PORT Pneumonia Outcomes Research Team, TEAE treatment-emergent AE *AEs with unknown start date, or partial date such that it could not be determined if they started on or after first study drug administration, were categorized as TEAEs; AEs were classified using the MedDRA version 20.0 † Related TEAEs were defined as TEAEs that were considered "definitely, " "probably, " or "possibly" related to study drug by the investigator. If the relationship for a TEAE was missing, it was considered "related. " Patients with multiple events in each category were counted only once in that category ‡ A patient could have > 1 TEAE leading to study drug discontinuation § Assessed in the intent-to-treat population (lefamulin, n = 646; moxifloxacin, n = 643) || Three patients in the lefamulin group had a TEAE leading to death after study substantial hepatotoxicity [35], these data show a favorable lefamulin safety profile, with low frequencies of transient hepatic enzyme elevations and TEAEs. Because the liver is a major elimination route for lefamulin (primarily via CYP3A4) [35], dosage adjustment is required for IV lefamulin in patients with severe hepatic impairment; oral lefamulin has not been studied in subjects with hepatic impairment and, based on available data, is not recommended in patients with moderate or severe hepatic impairment [19]. Study findings may be limited in their generalizability, as enrollment criteria may have excluded some patients typically seen in clinical practice such as those at risk for major cardiac events or dysfunction, patients with significant hepatic disease, and patients with severely impaired renal function. However, comorbidities common in patients presenting with CABP such as age, DM, a history of cardiovascular diseases (e.g., hypertension, CHF, or arrhythmia) or chronic lung diseases (e.g., asthma or COPD), elevated liver enzymes, or mild-to-moderate renal dysfunction were well represented in our analysis. Each of these comorbidities would be important for clinicians to consider when determining the intensity of care that a given patient with CABP will require. Similar to most contemporary antimicrobial clinical trials [36,37], enrollment in these studies was largely outside of the United States because of challenges associated with prestudy antibiotic treatment and hospitalization duration [38]. However, a recent FDA analysis of geographic differences across antimicrobial clinical trials from 2001 to 2017 indicated broad similarities in demographic, clinical, and microbiological characteristics, which lessens generalizability concerns for these trials [39]. Subgroup analysis findings are limited by relatively low sample size such that important but infrequent AEs may not have been detected and randomization may not have adequately balanced prognostic factors. Lastly, the data include some random variation owing to the number of subgroups examined.

Conclusions
In this pooled analysis of 2 pivotal CABP studies, clinical response rates were high and similar with lefamulin and moxifloxacin, achieving rapid clinical response that was sustained through LFU. Lefamulin was generally well tolerated regardless of administration route, suggesting a favorable benefit-risk profile. Subgroup analyses suggest lefamulin may be a promising empiric monotherapy option for both inpatients and outpatients with CABP, including patients presenting with advanced age or various comorbidities. In light of rising antibacterial resistance with macrolides, tolerability concerns with other antibiotic classes (eg, fluoroquinolones, beta-lactams), and/or failure of other antibiotic options, lefamulin may provide a much-needed IV/oral empiric monotherapy alternative.