Figure 2From: mAChRs activation induces epithelial-mesenchymal transition on lung epithelial cells TGF-β1-induced EMT is modulated by non-neuronal cholinergic system. Physostigmine (Physo, 100 μM) was added 1 h before stimulation with TGF-β1 (5 ng/mL) for 72 h. ACh in supernatants was determined with an LC–MS/MS assay. Cell lysates were assayed for EMT markers, ChAT and GAPDH. Representative western blots of E-cadherin (A), vimentin (B), α-SMA (C), ChAT (D) and GAPDH are shown. E: ACh level. Data are expressed as mean ± SEM of 4-6 independent experiments. # p < 0.05, ## p < 0.01 vs. control; *p < 0.05, **p < 0.01 vs. TGF-β1.Back to article page