Table 1 PCR mixtures and conditions used for the MIRU-VNTR genotyping
From: Unsuspected and extensive transmission of a drug-susceptible Mycobacterium tuberculosisstrain
Multiplex | Locus | Alias | VNTR length (bp) | [MgCl2] (mM) | PCR primer pairs (5' to 3'), with labelling indicated in bracketsa |
|---|---|---|---|---|---|
Mix 1 | 580 | MIRU 4 | 77 | 3 | GCGCGAGAGCCCGAACTGC (FAM) GCGCAGCAGAAACGCCAGC |
2996 | MIRU 26 | 51 | 3 | TAGGTCTACCGTCGAAATCTGTGAC CATAGGCGACCAGGCGAATAG (HEX) | |
802 | MIRU 40 | 54 | 3 | GGGTTGCTGGATGACAACGTGT (TAMRA) GGGTGATCTCGGCGAAATCAGATA | |
Mix 2 | 960 | MIRU 10 | 53 | 2 | GTTCTTGACCAACTGCAGTCGTCC GCCACCTTGGTGATCAGCTACCT (FAM) |
1644 | MIRU 16 | 53 | 2 | TCGGTGATCGGGTCCAGTCCAAGTA CCCGTCGTGCAGCCCTGGTAC (HEX) | |
3192 | MIRU 31 | 53 | 2 | ACTGATTGGCTTCATACGGCTTTA GTGCCGACGTGGTCTTGAT (TAMRA) | |
Mix 3 | 424 | Mtub04 | 51 | 1.5 | CTTGGCCGGCATCAAGCGCATTATT GGCAGCAGAGCCCGGGATTCTTC (FAM) |
577 | ETR C | 58 | 1.5 | CGAGAGTGGCAGTGGCGGTTATCT (HEX) AATGACTTGAACGCGCAAATTGTGA | |
2165 | ETR A | 75 | 1.5 | AAATCGGTCCCATCACCTTCTTAT (TAMRA) CGAAGCCTGGGGTGCCCGCGATTT | |
Mix 4 | 2401 | Mtub30 | 58 | 3 | CTTGAAGCCCCGGTCTCATCTGT (FAM) ACTTGAACCCCCACGCCCATTAGTA |
3690 | Mtub39 | 58 | 3 | CGGTGGAGGCGATGAACGTCTTC (HEX) TAGAGCGGCACGGGGGAAAGCTTAG | |
4156 | QUB-4156 | 59 | 3 | TGACCACGGATTGCTCTAGT GCCGGCGTCCATGTT (TAMRA) | |
Mix 5 | 2163b | QUB-11b | 69 | 1.5 | CGTAAGGGGGATGCGGGAAATAGG CGAAGTGAATGGTGGCAT (FAM) |
1955 | Mtub21 | 57 | 1.5 | AGATCCCAGTTGTCGTCGTC (HEX) CAACATCGCCTGGTTCTGTA | |
4052 | QUB-26 | 111 | 1.5 | AACGCTCAGCTGTCGGAT (TAMRA) CGGCCGTGCCGGCCAGGTCCTTCCCGAT |