Figure 1

Intracellular expression of IL-13 in CD3+ T cells. 1a: Representative histograms and dot plot of the percentage of CD3+ T cells expressing IL-13 in asthma, EB and healthy controls at baseline. Solid peak (isotype control), solid line (IL-13 FITC conjugate). 1b: Increased % expression by CD3 T cells of IL-13 in asthma at baseline compared to patients with EB and healthy subjects. *p < 0.05 (asthma subdivided into those with mild-moderate disease closed circles and severe asthma open circles). 1c: Upregulation of CD3+ T cell and eosinophil IL-13 expression in asthma after mitogen stimulation. Fold increase in IL-13 fluorescence compared to isotype control before and after mitogen stimulation.