Fig. 5

INSM1 ectopic expression suppresses cyclin D1 expression in bronchial epithelial cells. Immunohistochemical staining for cyclin D1, weaker and stronger positive signal were detected in the nuclei of bronchial epithelial cells in bi-transgenic mice (d-f) as compared to the control (a-c) on PN0, PN7, and PN21. Original magnification is 400X. g A human bronchial epithelial cell line, BEAS-2B, was infected with Ad-INSM1 or Ad-LacZ for 24 h and cultured in DMEM medium with 10 % fetal bovine serum for 14 or 24 h. The cell lysate was measured by Western blot analysis for INSM1 and cyclin D1 using housekeeping protein GAPDH as an internal control. Similar results were observed from three separated experiments