Fig. 1

Expression of oxidant/antioxidant factors in Mouse lung fibroblasts (MLF) incubated for 24 h with TGF-β1 and various concentrations of pirfenidone (PFD) for 48 h. MLF cells were stimulated with TGF-β1 (5 ng/mL) for 24 h before PFD (100 μg/mL, 200 μg/mL and 500 μg/mL) treatment for 48 h. The mRNA expression of nuclear factor-erythroid-related factor 2 (Nrf2), [BTB (broad-complex, tramtrack and bric-a-brac) and CNC (cap‘n’collar protein) homology 1, Bach1], heme oxygenase-1 (HO-1), and glutathione peroxidase 1 (GPx1) in MLF cells was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) (a, b, c, d). The protein levels of Nrf2, Bach1, Ho-1, and GPx1 in MLF cells were detected by Western blot (e). Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, vs. TGF-β1 group