Fig. 2

Effects of PFD on oxidant/antioxidant factors in Bleomycin (BLM)-induced pulmonary fibrosis in mice (3 in each group). BLM (5 mg/kg, intratracheally instillation for 14d) administered mice were then treated with PFD (300 mg/kg/d orally administered) for 4 weeks. Control group was intratracheally administered 50 μL 0.9% saline), only PFD group was orally administrated with 300 mg/kg PFD daily for 4 weeks. The mRNA expressions of Nrf2, Bach1, HO-1, and GPx1 in the lung were analyzed by RT-PCR (a, b, c, d); protein levels of Nrf2, Bach1, HO-1, and GPx1 in the lung were detected by Western blot (e). Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, vs. BLM group