Fig. 1

SDS-PAGE and western blot analysis of purified LAM-specific rabbit mAbs. (A) Recombinant antibodies LD9E3 and LD7C1 were tested by SDS–PAGE under non-reducing and reducing conditions. (B) Reactivities of rabbit anti-LAM mAbs to LAM antigens were tested by western blotting with 0.5, 1, 2 ug of LAM antigen and 10 ul crude TB LAM, respectively. M: Prestained protein ladder (10 to 250 kDa) was used. After electrophoretic transfer, a molecular weight marker was drawn on the membrane with the sole purpose of indicating the molecular size. N: negative control lane, bacterial E. coli lysate prepared in the same manner as the crude TB Lam sample