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Table 2 ECP primers and probes derived from the gene sequence of GenBank accession number X16545

From: Associations of ECP (eosinophil cationic protein)-gene polymorphisms to allergy, asthma, smoke habits and lung function in two Estonian and Swedish sub cohorts of the ECRHS II study

Polymorphism

Primer

Probea

ECP c.-38(A>C)

bFP: 5'-ATA GTT TTC ACC CAG AGT CCA-3'

 

(rs2233859)

bRP: 5'-TGC CCG CAT TGC AAT GGT GCA TCG A-3'

 
 

cFP: 5'-CAC CCA GAG TCC AGA TCC CAC CG-3'

 
 

cRP: 5'-GCA TTG CAA TGG TGC ATC GAG-3'

 

ECP c.-38(A>C)

FP: 5'-GCC TGT GGG TTG AGA CAC TAT AGA-3'

C: 5'-VIC-AGT AAC TAC TCC CCg ATC C-3'

 

RP: 5'-ATT TGG GAA GTG AAC AGT TTT GG-3'

A: 5'-6-FAM-AGT AAC TAC TCC CCt ATC C-3'

ECP434(G>C)

FP: 5'-GAG TAG ATT CCG GGT GCC TTT ACT-3'

G: 5'-VIC-AAA CTG CAg GTA TGC AGA-3'

(rs2073342)

RP: 5'-CGT GGA GAA TCC CGT GGA T-3'

C: 5'-6-FAM-AAA CTG CAc GTA TGC AG-3'

ECP562(G>C)d

FP: 5'-GGT TCC AGT TCA CCT GGA TAC C-3'

G: 5'-VIC-TCA GCA gTC CTC ATC-3'

(rs2233860)

RP: 5'-GGT ATG GAG ACT GAT GAG GAC AGT-3'

C: 5'-6-FAM-TCA GCA cTC CTC ATC-3'

ECP562(G>C)e

fFP: 5'-AAA TGC GAC CCC AGA GTG GCA-3'

 
 

fRP: 5'-AGG GGG AGA GAT TCA TGA TAA ACC C-3'

 
 

FP: 5'-GGT TCC AGT TCA CCT GGA TAC C-3'

G: 5'-VIC-TCA GCA gTC CTC ATC-3'

 

RP: 5'-TGA TTG AGG AGC TTG GCA GAT-3'

C: 5'-6-FAM-TCA GCA cTC CTC ATC-3'

  1. FP: forward primer; RP: reverse primer, a: Polymorphic bases are indicated by lower cases, b: PCR primers, c: sequencing primers, d: Method 1, e: Method 2, f: amplification of a fragment of the ECP gene