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Table 3 Tests for SP-A self-agglutination-assay dependency

From: Assessment of Surfactant Protein A (SP-A) dependent agglutination

Beads Goat-anti-human-SP-A antibody Anti-goat antibody Serum Ca2+ Agglutination
+ + + + - -
+ + + + + +
+ + + - + -
+ + + - - -
+ - + + + -
+ - - + + -
- + + + + -
+ + + SP-D - -
+ + + SP-D + -
+ + + - + with Mannose -
+ + + + + with Mannose -
+ + + +/without SP-D + +
+ + + +/+Triton X + -
  1. The table shows twelve conditions of the bead assay with varying reagents: beads, anti-goat antibody, goat-anti-human antibody, human serum and calcium ions (Ca2+). The "+" means that the reagent was used for incubation. Without SP-A and/or calcium ions in serum, and also with Mannose no agglutination was detected. The experiments were repeated three times. For the last condition a concentration of 0.1% (v/v) Triton X-100 was used (0.3 μl Triton X-100 in 1 ml pure water, 3 μl of this water were used for the assay). A final concentration of 100 ng/ml SP-A (1 ng) in test serum and 100 ng/ml SP-D standard (1 ng recombinant human SP-D) were used.