Figure 3

Gene expression measured downstream NPS stimulation of transiently NPSR1-A or NPSR1-B overexpressing HEK-293 cells. (a) NPSR1-A and -B differentially expressed genes as assessed using affymetrix expression-arrays revealed 66 genes induced by the -A isoform (cut off = ≥2 fold), of which 44 were induced by -B. No unique genes were induced by the -B isoforms. Genes are sorted by fold change, starting with the most differentially expressed gene as 1 (the numbers on the x-axis correspond to the order of the genes depicted in table 1). (b) qRT-PCR verification showing relative expression of CGA, CD69 and NTS after NPS-NPSR1 concentration- and time-response experiments. CGA, representing the majority of the genes in the expression-array experiment, demonstrate NPSR1-A as an isoform that induces stronger signalling effects both for NPS concentration-response and time-response. CD69 was the only gene greater induced by NPSR1-B and NTS did not respond to NPSR1-B signalling. Data shown as means relative to an NPS stimulated empty vector control ± SEM. * Indicates at which concentrations or time points the difference between NPSR1-A and -B was significant (p ≤ 0.05).