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Table 5 Summary table of the technical validation of ELM

From: Measurement of MMP-9 and -12 degraded elastin (ELM) provides unique information on lung tissue degradation

Technical validation step

ELN-441

Target

MMP degradation of human elastin

Detection range

0,484–125 ng/mL

Dilution range of serum samples

1:2, 1:3 and 1:4 is recommended

Dilution range of plasma samples

1:2, 1:3 and 1:4 is recommended

Dilution recovery of human serum*

91%

Dilution recovery of human plasma*

95%

Intra-assay variation**

9.44%

Inter-assay variation**

13.8%

Analyte stability***

103%

  1. *Percentage dilution recovery was calculated as the mean of 5 human samples diluted 1:2 and 1:4. **Inter- and intra-assay validation was calculated as the mean variation between 8 individual determinations of each human serum sample. ***The stability of the analyte (human serum) was calculated as the mean of three different serum samples were tested after freeze/thaw for one to 10 times.
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BMC Pulmonary Medicine

ISSN: 1471-2466

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