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Table 2 Sputum composition (percentage of sputum leukocytes and cell count)

From: Low-dose endotoxin inhalation in healthy volunteers - a challenge model for early clinical drug development

 

ANOVA

 

Baseline

LPS 1

LPS 2

LPS Tx

p

Macrophages (%)§

68.7 ± 5.3

19.1 ± 2.9***

25.9 ± 3.5***

27 ± 4.5***

<0.001

Neutrophils (%)§

25.2 ± 5.0

72.3 ± 3.4***

61.5 ± 3.5***

61.8 ± 4.6*** #

<0.001

Eosinophils (%)§

0.0 ± 0.0

0.1 ± 0.0

0.2 ± 0.1

0.1 ± 0.1

n.s.

Lymphocytes (%)§

0.7 ± 0.2

0.5 ± 0.2

0.5 ± 0.2

0.4 ± 0.1

n.s.

Monocytes/sm. MФ (%)§

5.3 ± 0.9

7.9 ± 1.4

11.9 ± 1.5*** #

10.7 ± 0.9**

0.001

Cummulative Response (%)§

30.5 ± 5.4

80.3 ± 3.0***

73.3 ± 3.5***

72.5 ± 4.5***

<0.001

Sq. cells (% TCC)&

7.7 (1.4)

2.9 (1.2)***

2.5 (1.4)***

2.8 ± 1.3***

<0.001

Total cell count (106/mL)&

2.40 (1.2)

10.46 (1.2)***

5.08 (1.2)** ##

3.7 (1.2)*###

<0.001

Macrophages (106/mL)&

1.43 (1.2)

1.73 (1.2)

1.11 (1.3)

0.80 (1.2)* #

0.02

Neutrophils (106/mL)&

0.39 (1.5)

7.29 (1.3)***

2.91 (1.3)***##

2.10 (1.2)***###

<0.001

Non-Sq. epithelia cells (106/mL)&

0.15 (1.4)

0.12 (1.5)

0.14 (1.5)

0.09 (1.4)

n.s.

Monocytes/sm. MФ (106/mL)&

0.10 (1.3)

0.66 (1.4)***

0.52 (1.3)***

0.36 (1.2)***

<0.001

Cummulative Response (106/mL)&

0.53 (1.4)

8.13 (1.2)***

3.47 (1.2)***##

2.51 (1.2)***###

<0.001

  1. § mean and standard error of the mean (SEM) of percent sputum leukocytes, & geometric mean and geometric standard error (SEM as a factor), TCC: total cell count; Sq: Squamous; sm. MФ: small macrophages; ANOVA: repeated measures ANOVA, Newman-Keuls post-hoc test: * p < 0.05, ** p < 0.01, *** p < 0.001 compared with baseline; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with LPS 1. Cumulative response = Sum of neutrophils, monocytes and small macrophages.
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BMC Pulmonary Medicine

ISSN: 1471-2466

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