Figure 1
From: Obesity increases eosinophil activity in asthmatic children and adolescents
Human eosinophil chemotaxis in asthmatic obese (AO), asthmatic non-obese (ANO), non-asthmatic obese (NAO) and non-asthmatic non-obese (NANO) subjects in response to (A) minimum essential medium (MEM, 27 μL), (B) eotaxin (300 ng/ml), (C) PAF (10 μM) and (D) RANTES (100 ng/ml). Eosinophils (4 × 106/ml /50 μl / well) were incubated for 1 h at 37°C in the microchemotaxis chamber, and the filters were stained and mounted on a glass slide. Eosinophil migration was determined after counting the eosinophils that migrated completely through the filter in five random high-powered fields (HPF; 1000 x) per well. The results are expressed as the mean migrated cells for each individual (P values are shown in figure).