Figure 1
From: Bone marrow-derived progenitor cells in end-stage lung disease patients
Identification of progenitor populations. Taqman PCR measurement of (A) Clara Cell Secretory Protein (CCSP) gene expression in human bone marrow cells (BMCs), (B) peripheral blood mononuclear cells (PBMCs), and (C) PBMCs pre-sorted for CCSP by flow cytometry. No template and no reverse transcription negative controls are included. (D) Gel electrophoresis of PCR product after Taqman-based amplification. Positive control (bronchus) and negative no reverse transcription (NRT) controls are included. (E) Flow cytometry gating for measurement of CCSP+ PBMCs based on isotype control staining. (F) Flow cytometry gating for measurement of CD45+Collagen-1+ peripheral blood leukocytes (PBLs) based on isotype control staining.