Figure 1

Differential NOD1 expression and response patterns between differentiated macrophages and monocytes. Unstimulated alveolar macrophages (AMs), monocyte-derived macrophages (MDMs) and monocytes (MNs) were lysed. NOD1 protein expression was measured from the cytosolic fractions by western blot analysis; one representative experiment out of three is depicted (A) and the mean ± SE of protein expression after normalized to tubulin is depicted, n = 3 (B). Total RNA was extracted from cell lysates and reverse transcribed. NOD1 gene expression is depicted relative to the 18S RNA content of at least 9 subjects; the lines represent the median values (C). Cells were incubated in the presence or absence of 5 μg/ml of Tri-DAP for 24 h. Up-regulation of NOD1 gene expression was assessed by quantitative PCR using the Taqman system and ΔΔCT for relative quantification. The fold changes of gene expression relative to the unstimulated cells of at least 9 subjects are depicted; the lines indicate the median values; *p < 0.05, Mann Whitney U test (D).