Figure 2

Macrophages stimulated with Tri-DAP release proinflammatory cytokines. Cells were incubated in the presence or absence of 5 μg/ml of Tri-DAP (n = 10, A) or 100 ng/ml of LPS (n = 8, B). The supernatants were collected after 24 h; cytokine production was measured using milliplex technology. Box plots indicate median and quartiles, *p < 0.05, **p < 0.01, ns = not significant Wilcoxon Rank test.