Figure 6

Tri-DAP treatment contributes to intracellular growth control of mycobacteria in alveolar macrophages. AMs were infected with M. tuberculosis H37Rv using an infection ratio of 1–2 bacteria/20 macrophages for 1 h. Non-phagocytized bacteria were washed away. Then, the macrophages were treated with 5 μg/ml of Tri-DAP for 24 h. The intracellular bacterial burden was measured by quantifying the colony-forming units, and the intracellular growth index was calculated after 1 and 4 days post-infection relative to the phagocytized bacteria at day 0; n = 7, *p < 0.05 (A). TNFα production was determined by ELISA in 24 h supernatants, n = 7, *p < 0.05, box plots indicate median and quartiles (B). LC3 gene expression was measured by quantitative PCR using the Taqman system and ΔΔCT method for relative quantification. The fold changes in gene expression relative to the unstimulated cells of at least 8 subjects are depicted; bold lines indicate medians (C).