Figure 3

Effect of acrolein and nicotine on caspase-3 activation in PNEC cultures determined using western blotting. Western blots of cleaved caspase 3 in healthy PNECs (with beta-actin loading controls). Blot A: lanes 1–3 represent treatment of PNECs with 0, 5, 50 μM nicotine (1 h); blot B: lanes 1–3: 0, 10, 50 μM acrolein (1 h); blot C: lanes 1–3 represent treatment of PNECs with 0, 5, 50 μM acetylaldehyde (1 h) and blot D lanes 1–3: 0%, 5%, 50% CSE (1 h) respectively.