Figure 4

Effect of acrolein, nicotine, acetylaldehyde and CSE treatment on cell viability. In each plot, the horizontal axis represents intensity of staining for Annexin V and vertical axis intensity of staining for PI (determined in the FL1 and FL3 plot respectively, both logarithmic scale). Dot plots represent (A) untreated cells, (B) cells treated with Triton-X for 4 h, (C) cells treated with 5 μM staurosporin for 4 h, (D) cells treated with media alone for 4 h, (E) cells treated with 50 μM nicotine for 24 h, (F) cells treated with 50 μM acrolein for 4 h, (G) cells treated with 50 μM acetylaldehyde for 24 h, and (H) cells treated with 5% CSE for 24 h.