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Table 1 Annexin-V/Propidium iodide analysis of acrolein, nicotine, acetylaldehyde and CSE treatment in PNEC cultures

From: Inflammatory and cytotoxic effects of acrolein, nicotine, acetylaldehyde and cigarette smoke extract on human nasal epithelial cells

 

Av (-) PI (-)

Av (+) PI (-)

Av (+) PI (+)

Av (-) PI (+)

Media 24 h

99.1%

0.4%

0.4%

0.1%

Nicotine 24 h

39.7%

45.5%

13.0%

1.8%

Acrolein 4 h

26.3%

2.2%

39.8%

31.7%

Acetylaldehye 24 h

97.4%

0.2%

2.0%

0.4%

5% CSE 24 h

51.8%

19.1%

25.6%

3.5%

  1. Percentages of events positive for apoptosis and necrosis are shown corresponding to dot-plots D-H in Figure 4.