Figure 4From: Long-term exposure to muscarinic agonists decreases expression of contractile proteins and responsiveness of rabbit tracheal smooth muscle cells Muscarinic agonists reduce the expression of contractile phenotype marker smooth muscle myosin heavy chain (SM-MHC) in ASMCs. A. Western blot analysis with anti-SM-MHC and anti-β-actin antibodies in total protein extracts from ASMCs incubated for 15 days in serum free medium (control) or in medium containing 10% FBS, ACh (10-5 M) or CCh (3 × 10-7 M). B. Quantification of SM-MHC expression normalized to the expression of β-actin presented in A. C. Specific SM-MHC expression by indirect immunofluorescence in ASMCs incubated for 15 days in serum free medium (control) or in medium containing 10% FBS, ACh (10-5 M) or CCh (3 × 10-7 M). Nuclei were stained with DAPI (lower panel). D. Counting of the percentage of rabbit tracheal smooth muscle cells which express SM-MHC in C. Ν = 4 independent experiments. *P < 0.05 compared with control.Back to article page