Figure 5
The effect of muscarinic agonists ACh and CCh on N-methyl-[ 3 H]scopolamine ([ 3 H]NMS) specific binding. Rabbit tracheal ASMCs were incubated for 3-15 days in serum free medium (control) or medium containing 10% FBS, ACh (10-5 M) or CCh (3 × 10-7 M). Specific binding was calculated using 5 nM of [3H]NMS in triplicate for each condition. #P < 0.05 compared with cells serum starved for 3 days. *P < 0.05 compared with the respective control.