Figure 8
Muscarinic agonists increase ASMCs number. Rabbit tracheal ASMCs were incubated for 7 days in serum free medium before the addition of the muscarinic agonist in the culture medium for the indicated time. A. The effect of 1-5 days of incubation with 10-5 M ACh or 3 × 10-7 M CCh on rabbit tracheal smooth muscle cell number estimated with the MTT-Cell Titer 96® AQueous One Solution Assay (Promega) method. B. The effect of 3-15 days of incubation with 10-5 M ACh or 3 × 10-7 M CCh on rabbit tracheal smooth muscle cell number estimated with Trypan blue method. *P < 0.05 and **P < 0.01 compared with the respective control.