Figure 9
The involvement of PI3K and MAPK signalling pathways on the muscarinic agonists induced proliferation of ASMCs. Rabbit tracheal ASMCs were incubated for 7 days in serum free medium before the addition of either FBS (10%), ACh (10-5 M) or CCh (3 × 10-7 M) for 24 h with or without either PD98059 (100 μM) in A or LY294002 (20 μM) in B. **P < 0.01 compared with control. #P < 0.05 and ##P < 0.01 compared with the effect of control, 10% FBS or muscarinic agonist alone on cell number.