Figure 5

Vβ segments with absolute usage difference between BAL cells and PBMC. Each dot indicates difference, BAL vs. PBMC, in one Vβ segments in one patient. The expression (% of the gated population) in PBMC was subtracted from the expression in BAL. Positive values indicate a greater expression in BAL and negative values indicate a greater expression in PBMC. The arbitrary cut off > 5% difference is indicated by a dotted line. A) In CD4⁺ T cells 9 Vβ segments had > 5% higher or lower expression in BAL cells versus PBMC in at least one patient. Segments with higher expression in BAL were Vβ1, Vβ17 and Vβ22, while segments Vβ3, Vβ8, Vβ13.1 had higher expression in PBMC. Segment Vβ2 had higher expression in BAL in one patient and higher expression in PBMC in two other patients; Vβ5.1 had higher expression in BAL in one patient and in PBMC in another patient. B) In CD8⁺ T cells 10 Vβ segments had > 5% difference in expression comparing BAL cells and PBMC in at least one patient. Vβ1 and Vβ2 both had higher expression in BAL from two patients and in PBMC in one patient. Vβ7.1 and Vβ7.2 had higher expression in BAL in one patient each; Vβ8, Vβ13.2 and Vβ16 had higher expression in BAL in one patient and higher expression in PBMC in another patient each, and the segments Vβ11, Vβ12 and Vβ13.1 had higher expression in PBMC in one patient each.