Figure 4

Distribution and expression of occludin (immunohistochemistry, ×400). Immunohistochemical staining for occludin protein in vascular endothelial cells and alveolar epithelial cells in paraffin sections of rat lungs 4 h after ventilation. (a) control group, (b) low tidal volume group, (c) low tidal volume + PKC inhibitors group, (d) high tidal volume group, (e) high tidal volume + PKC inhibitors group. The low tidal volume was 7 ml/kg; the high tidal volume was 42 ml/kg. Brown particles represent occludin-positive expression. Data are representative of three independent experiments.