Figure 5

Effects of ventilation and the PKC inhibitor BIM on occludin expression in rats. Rats were subjected to the low tidal volume (7 ml/kg) or the high tidal volume (42 ml/kg) for 4 h. Groups L + P and H + P were pretreated with BIM 0.12 mg/kg 1 h before ventilation. After experiments, the rats were killed and occludin protein expression level was determined by Western blot analysis. The density of protein in the control group was used as a standard to compare relative densities in the other groups. *P <0.05 vs. control; #P <0.05 vs. group L; & p < 0.05 vs. group H. Data are representative of three independent experiments. A P value <0.05 indicates statistical significance.